{"id":104059,"date":"2018-10-11T21:30:02","date_gmt":"2018-10-11T21:30:02","guid":{"rendered":"http:\/\/news.alabamanewsreporter.com\/story\/104059\/exkine-nuclear-and-cytoplasmic-protein-extraction-kit-from-abbkine.html"},"modified":"2018-10-11T21:30:02","modified_gmt":"2018-10-11T21:30:02","slug":"exkine-nuclear-and-cytoplasmic-protein-extraction-kit-from-abbkine","status":"publish","type":"post","link":"https:\/\/www.alaskanewsdesk.com\/news\/story\/104059\/exkine-nuclear-and-cytoplasmic-protein-extraction-kit-from-abbkine.html","title":{"rendered":"ExKine\u2122 Nuclear and Cytoplasmic Protein Extraction Kit from Abbkine"},"content":{"rendered":"
1. ExKine\u2122 Nuclear and Cytoplasmic Protein Extraction Kit is suitable for fresh mammalian cultured cells and tissues with little or no cross-contaminations.<\/p>\n

2. non-denatured, active proteins are purified in less than two hours.<\/p>\n

3. apply in various downstream assays, including Western blotting, gel-shift assays, protein assays, reporter gene assays and enzyme activity assays.<\/p><\/div>\n

Abbkine ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit<\/a> enable stepwise separation and preparation of crude cytoplasmic and nuclear extracts from mammalian cultured cells or tissue.<\/p>\n

The Nuclear and Cytoplasmic Protein Extraction Kit contains Cytoplasmic Solution A (CES A), Cytoplasmic Solution B (CES B), Nuclear Extraction Solution (NES), DTT (500X), Protease Inhibitor (100X).<\/p>\n

The Protein Extraction Kit is based on allowing cells to swell with hypotonic buffer. And then the cells are disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer. Non-denatured, active proteins are purified in less than two hours.<\/p>\n

\"\"<\/a>Sample: 293T cells lysate.<\/p>\n

C: cytoplasmic extraction was analyzed using α-tubulin antibody (A01080<\/a>).<\/p>\n

N: nuclear extraction was analyzed using α-tubulin antibody (A01080).<\/p>\n

The Nuclear and Cytoplasmic Protein Extraction Kit<\/a> has below three features:<\/p>\n

1. Versatile — suitable for fresh mammalian cultured cells and tissues with little or no cross-contaminations.<\/p>\n

2. Fast and convenient — non-denatured, active proteins are purified in less than two hours. <\/p>\n

3. Compatible — apply in various downstream assays, including Western blotting, gel-shift assays, protein assays, reporter gene assays and enzyme activity assays.<\/p>\n

More details of Abbkine’s ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit, please click KTP3001<\/a>.<\/p>\n

Media Contact<\/span>
Company Name:<\/strong> Abbkine Scientific<\/a>
Contact Person:<\/strong> Media Relations
Email:<\/strong> Send Email<\/a>
Phone:<\/strong> +86-27-59716789
Address:<\/strong>Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave
City:<\/strong> Wuhan
State:<\/strong> Hubei
Country:<\/strong> China
Website:<\/strong> https:\/\/www.abbkine.com\/<\/a><\/p>\n

\"\"<\/p>\n","protected":false},"excerpt":{"rendered":"

1. ExKine\u2122 Nuclear and Cytoplasmic Protein Extraction Kit is suitable for fresh mammalian cultured cells and tissues with little or no cross-contaminations. 2. non-denatured, active proteins are purified in less<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/www.alaskanewsdesk.com\/news\/wp-json\/wp\/v2\/posts\/104059"}],"collection":[{"href":"https:\/\/www.alaskanewsdesk.com\/news\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.alaskanewsdesk.com\/news\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.alaskanewsdesk.com\/news\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.alaskanewsdesk.com\/news\/wp-json\/wp\/v2\/comments?post=104059"}],"version-history":[{"count":0,"href":"https:\/\/www.alaskanewsdesk.com\/news\/wp-json\/wp\/v2\/posts\/104059\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.alaskanewsdesk.com\/news\/wp-json\/wp\/v2\/media?parent=104059"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.alaskanewsdesk.com\/news\/wp-json\/wp\/v2\/categories?post=104059"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.alaskanewsdesk.com\/news\/wp-json\/wp\/v2\/tags?post=104059"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}